Signal amplification of padlock probes by rolling circle replication
نویسندگان
چکیده
منابع مشابه
Signal amplification of padlock probes by rolling circle replication.
Circularizing oligonucleotide probes (padlock probes) have the potential to detect sets of gene sequences with high specificity and excellent selectivity for sequence variants, but sensitivity of detection has been limiting. By using a rolling circle replication (RCR) mechanism, circularized but not unreacted probes can yield a powerful signal amplification. We demonstrate here that in order fo...
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ABBREVIATIONS bp base pair cDNA complementary DNA CV coefficient of variation DNA deoxyribonucleic acid FRET fluorescence resonance energy transfer HRCA hyperbranched rolling circle amplification LN lymph node nt nucleotide OLA oligonucleotide ligation assay PCR polymerase chain reaction RCA rolling circle amplification RCR rolling circle replication RNA ribonucleic acid SCA serial circle ampli...
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While microarrays hold considerable promise in large-scale biology on account of their massively parallel analytical nature, there is a need for compatible signal amplification procedures to increase sensitivity without loss of multiplexing. Rolling circle amplification (RCA) is a molecular amplification method with the unique property of product localization. This report describes the applicat...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/26.22.5073